American Society of Hematology

Figure 2

Alloactivated Treg cells express granzyme B in a mouse model of GVHD in vivo. Lethally irradiated Balb/c mice were reconstituted with 2 × 106 bone marrow cells and 2 × 106 T cells derived from 129/SvJ WT mice. Splenocytes were harvested from recipient mice on days 3 to 6 for flow cytometric analysis of granzyme B expression in donor-derived Treg cells. (A) Representative flow plots, gated on H-2Kb+Foxp3+ Treg cells, are shown. (B) Summary graph of percentage granzyme B–expressing Treg cells is shown (n = 3 mice per time point).

Alloactivated T_reg cells express granzyme B in a mouse model of GVHD in vivo. Lethally irradiated Balb/c mice were reconstituted with 2 × 10⁶ bone marrow cells and 2 × 10⁶ T cells derived from 129/SvJ WT mice. Splenocytes were harvested from recipient mice on days 3 to 6 for flow cytometric analysis of granzyme B expression in donor-derived T_reg cells. (A) Representative flow plots, gated on H-2K^b+Foxp3⁺ T_reg cells, are shown. (B) Summary graph of percentage granzyme B–expressing T_reg cells is shown (n = 3 mice per time point).

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