Similar differentiation capacity of WT and mib mutant EMPs in vitro.lmo2^hi, gata1⁺ 30-hpf EMPs from WT and mib embryos were isolated by FACS and plated on zebrafish kidney stroma (ZKS). Samples from WT (A) and mib (B) cultured cells were cytocentrifuged and stained after 3 (3 left columns) or 5 (3 right columns) days in culture. Cells were stained for morphology with May-Grünwald/Giemsa (2 left and 2 right columns). o-Dianisidine, a chemical stain for hemoglobin, was used to assess erythroid differentiation (middle column). (C) Differential cell counts for immature blasts (yellow), erythroid (red), and myeloid (blue) cell types yielded statistically similar percentages from WT or mib EMPs (*P = .988; **P = .802). The y-axis indicates percentages of cultured cells. Levels reflect the average of 3 experiments. Statistical analyses were performed by a 2-sample, unequal-variance t test with 2-tailed distribution.