Lack of Notch signaling does not affect EMP number or gene expression. (A) lmo2^higata1⁺ dual-positive cells (EMPs) are present in wild-type (top left) and mindbomb (top right) embryos at 30 hpf. EMPs (in boxed gate) are a fraction of the total live cells in the embryo at this time point. Resorting shows > 97% purity of isolated EMP populations (bottom row). (B) Gene expression of sorted 28-30 hpf WT and mib EMPs was examined by qPCR and compared with WKM as a reference. HSC (runx1), thrombocytic (cd41), erythroid (gata1), and myeloid (pu.1, mpx) transcript levels were determined. The y-axis indicates expression relative to WKM, defined as 100% in all examples. Levels reflect the average of 3 experiments; error bars represent standard deviation.