EMPs, but not HSCs, are specified in mib mutant embryos. Expression of the HSC transcripts cmyb in wild-type (Ai) and mib (Aii) and runx1 in wild-type (Bi) and mib (Bii) at 30 hpf. Expression of the EMP transcript pu.1 at 24 hpf in wild-type (Ci) and mib (Cii) and 26 hpf in wild-type (Di) and mib (Dii) and mpx at 36 hpf in wild-type (Ei) and mib (Eii) and 48 hpf in wild-type (Fi) and mib (Fii). Embryos are shown with anterior to the left and dorsal up. Dashed line boxes describe the embryonic regions (AGM: Ai-ii, Bi-ii; PBI: Ci-ii, Di-ii; and CHT: Ei-ii, Fi-ii) shown in magnified images to the right of each whole embryo view. Magnification: ×100 in whole embryo images; ×300 in AGM, PBI, and CHT images.