Severe sepsis impaired effective antitumor responses. (A) At day 15 post septic and sham mice were inoculated subcutaneously in the flank with 0.5 × 106 LLC cells, and tumor growth was monitored at various time points as indicated. Thus, day 15 is 30 days after CLP or sham surgery. Data shown are the results from 3 independent experiments. Horizontal lines indicate the comparison between sham and post-CLP mice at the indicated times after tumor injection. ns indicates not statistically significant. (B-C) IFN-γ, IL-17, TNF-α, and CCL5 were measured in tumor homogenates at days 11 and 20 after LLC injection with the use of Bioplex. (A) Pooled results from 3 independent experiments are shown. (B-C) Data represent the mean ± SEM from 5 to 7 individual mice per group. *P ≤ .05; ***P ≤ .001 when post septic mice were compared with sham mice bearing tumors.
