Analysis of BHK cells harboring WT β3 with WT αIIb or αIIb with substitutions of Trp110 or Phe171 by hydrophobic residues. (A) Flow cytometry of cells harboring Trp110Leu or Trp110Arg exhibited surface expression of αIIbβ3 and fibrinogen binding that was not significantly different from WT cells, whereas Phe171Ile and Phe171Val mutants exhibited reduced αIIbβ3 expression and fibrinogen binding. Binding is shown as mean percentage of WT MFI ± SD (mean of at least 3 different transfections). *P < .05. (B) Immunoblotting of cell lysates on Tris-acetate 3% to 8% gels developed with monoclonal antibody against αIIb (SZ22). Percentage mature αIIb of total αIIb was calculated by measuring band intensity for each cell line using the EZQuant densitometry program.