Comparisons of blast percentages in the bone marrow and peripheral blood leukocyte counts between mice receiving KSLs transduced with BCR-ABL alone and those receiving KSLs, CMPs, and GMPs transduced with the combination of Hes1 and BCR-ABL. (A) Blast ratios in the bone marrow. The mean blast ratios in all nucleated bone marrow cells were 6.5% ± 0.7% and 36.5% ± 6.9% in mice receiving KSLs transduced with BCR-ABL alone and in those receiving KSLs, CMPs, and GMPs transduced with the combination of Hes1 and BCR-ABL, respectively. The difference was statistically significant by the 2-sample t test with Welch correction (P < .001). (B) Peripheral white blood cell counts (WBCs). WBCs were 3.4 ± 0.6 × 104/μL and 17.8 ± 20.3 × 104/μL in mice receiving KSLs transduced with BCR-ABL alone and in those receiving KSLs, CMPs, and GMPs transduced with the combination of Hes1 and BCR-ABL, respectively. The difference was statistically significant by the 2-sample t test with Welch correction (P < .001). (C) Flow-cytometric analysis of bone marrow cells from mice receiving GMPs transduced with the combination of Hes1 and BCR-ABL. The dot plots represent Gr-1, CD11b, c-Kit, Sca-1, CD3, CD4, CD8a, B220, CD19, CD34, Ter119, and NGFR labeled with the corresponding PE-conjugated monoclonal antibody versus expression of GFP/BCR-ABL. NGFR is a marker of Hes1, and GFP is a marker of BCR-ABL transduction. The bone marrow cells derived from mice receiving KSLs or CMPs transduced with the combination of Hes1 and BCR-ABL showed essentially the same pattern (supplemental Figure 5A-B).
