Wiskostatin inhibits chemokine-induced LFA-1 high-affinity state. Freshly isolated peripheral blood NK cells were preincubated for 1 hour with wiskostatin (10μM) or vehicle (DMSO) and then stimulated with CX3CL1/fractalkine (1nM) or CXCL12/SDF-1 (10nM) and simultaneously stained with the high-affinity reporter mAb 327C for the indicated time periods at 37°C. Stained cells were then analyzed by flow cytometry. The ratio between the mean fluorescence intensity of stimulated (open profile) versus unstimulated (black profile) cells is shown. These results represent 1 of 3 independent experiments.