Apelin suppresses VEGF-induced vascular edema. (A) Histologic analysis of hind limb muscles 14 days after resection of the femoral artery and gene therapy as indicated. (B) Miles assay using VEGF and apelin. Left-hand panels: Representative images of the vascular leakage induced by VEGF in the presence or absence of apelin (1 or 10 ng/mL). PBS was used as a negative control. Right-hand panel: Evans blue dye content eluted from dissected skin (mean ± SEM; n = 10). *P < .01.