Figure 5
![Figure 5. HJV/RGMc expression in mouse liver. (A-B) In situ hybridization analysis of HJV mRNA expression in mouse (C57BL/6) livers at different age. Note that only antisense (A), but not sense (B), HJV probes showed signals, demonstrating the specificity. (C) Confocal images of coimmunohistochemical detection of both HJV and GS, a marker of hepatocytes surrounding pericentral regions, in liver sections of P25 wild-type mouse. Arrows point to HJV-expressing hepatocytes. Central veins (CVs) and portal tract (PT) are indicated; bars represent 100 μm[b]. (D) Illustration of HJV “zonal distribution” pattern (pink color). In panels A through C, 3 different wild-type mice for indicated age were examined, and representative images are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/15/10.1182_blood-2009-11-251199/4/zh89991049780005.jpeg?Expires=1722765918&Signature=m-ir8dmYRd34lbzjr~~oHZ5mBAcLm0X~VaI8KQ38qwwc14W6MwXUb9QLsdUmPTrO~-A9mEYpzyvjV2bCOIPKFAQIauw6PHHEwgpCKsCgOqWRj56BR0mBNq7fnX9HXkJHvbbxenTBkNYeBveMbqkTFCSNd8kdjdzfP~WQm2wV2ifL0MLXeEYkTXTWZPP01jsyjIJnaWY7XBWUaBmLzOw7METuqA3kGrdJCkbzPxjiERwXS6Ypn850CJ7LOxiyRWquTti9OxBfD7mpb-l0Ama2FDeoRgld1E7-UtymmVvuHDejSQGCzs5OcZPuBeOY2aXyTtaAZsu8qeqJapURkK0WGQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HJV/RGMc expression in mouse liver. (A-B) In situ hybridization analysis of HJV mRNA expression in mouse (C57BL/6) livers at different age. Note that only antisense (A), but not sense (B), HJV probes showed signals, demonstrating the specificity. (C) Confocal images of coimmunohistochemical detection of both HJV and GS, a marker of hepatocytes surrounding pericentral regions, in liver sections of P25 wild-type mouse. Arrows point to HJV-expressing hepatocytes. Central veins (CVs) and portal tract (PT) are indicated; bars represent 100 μm[b]. (D) Illustration of HJV “zonal distribution” pattern (pink color). In panels A through C, 3 different wild-type mice for indicated age were examined, and representative images are shown.
