12/15-LOX^−/− mice show lack of angiogenic response to AA. (A-B) Wild-type and 12/15-LOX^−/− mice were injected subcutaneously with 0.5 mL of Matrigel premixed with vehicle, 5μM AA, or 5μM 15(S)-HETE as indicated. One week later, the animals were sacrificed, and the Matrigel plugs were harvested from underneath the skin and either processed for vWF and CD31 by double immunofluorescence staining by the use of their specific antibodies (A) or analyzed for hemoglobin content with Drabkin reagent (B). (C) Wild-type and 12/15-LOX^−/− mice were injected subcutaneously with 0.5 mL of Matrigel premixed with vehicle or 5μM AA. One week later, the Matrigel plugs were retrieved from animals, minced, incubated with 10μM AA and 10μM ionomycin, and 15(S)-HETE production was measured by EIA. The bar graphs represent the quantitative analysis of 6 plugs from 6 animals. The values are presented as the mean ± SD. *P < .01 vs WT control. **P < .01 vs WT control or 12/15-LOX^−/− control or 12/15-LOX^−/− + AA.