FGF-2 mediates 15(S)-HETE–induced angiogenesis. (A-B) Quiescent HDMVECs were treated with normal serum or neutralizing anti–FGF-2 antibodies (3 μg/mL) for 30 minutes at 37°C followed by washing with medium 131. The cells were then subjected to 15(S)-HETE (0.1μM)–induced migration (A) or tube-like structure formation (B) in the presence and absence of 3 μg/mL of preimmune serum or neutralizing anti–FGF-2 antibodies. (C) C57BL/6 mice were injected subcutaneously with 0.5 mL of Matrigel premixed with vehicle or 5μM 15(S)-HETE in combination with 20 μg/mL preimmune serum or neutralizing anti–FGF-2 antibodies. One week later, the animals were sacrificed, and the Matrigel plugs were harvested from underneath the skin and either processed for vWF and CD31 expression by double immunofluorescence staining using their specific antibodies or analyzed for hemoglobin content using Drabkin reagent. The bar graphs represent the quantitative analysis of 3 independent experiments or 6 plugs from 6 animals. The values are presented as the mean ± SD. *P < .01 vs control (normal serum); **P < .01 vs 15(S)-HETE (normal serum + 15(S)-HETE).