Figure 5
![Figure 5. E-selectin engagement induces phosphorylation of Btk, PLCγ2, and PI3K. Bone marrow–derived neutrophils were plated on uncoated (unstimulated) or E-selectin–coated wells for 10 minutes, and then lysates were prepared. (A) Lysates were immunoprecipitated with anti-Btk, followed by immunoblotting (IB) with a general phosphotyrosine (PY; 4G10) antibody. (B) Lysates were immunoblotted with antibody to phosphorylated PLCγ2 (phospho-PLCγ2 [Tyr1217]), total PLCγ2 (n = 3), phosphorylated Akt (n = 3), total Akt (n = 3), phosphorylated p38 MAPK (phospho-p38), or total p38 (n = 3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/15/10.1182_blood-2009-11-254185/4/zh89991051370005.jpeg?Expires=1723911666&Signature=vn6ypEE7N94St05MEjgaCM7fABz3TojynyPhY1I4x0ASQ9k81eemUKHrd2HtbuPQblwj7PFMmSm0waFDUlG3XtgM1P5LytWj2YeXAGaBsiJor2nOPsZujXwMnlrNAHXCAToywIzCBX1zAG7PEByF~uprMmocN13dXc7cvPSbSz8IqJ7Q7REP9R4nPsXt9IvJSuuCyQrwmu7cKMIkO6D1uhqojop~Ddz9HfX7dWxuyR0U~uELSVcfIItrlWWlMJI2kdiCGDcIBNYi~Vqinj7XVk9aJb5pCOBFePqgryVw2tkNNaC4tG6nLF8o4Nx5WhBvMu-3PD-CPofEFImzOaDRyg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
E-selectin engagement induces phosphorylation of Btk, PLCγ2, and PI3K. Bone marrow–derived neutrophils were plated on uncoated (unstimulated) or E-selectin–coated wells for 10 minutes, and then lysates were prepared. (A) Lysates were immunoprecipitated with anti-Btk, followed by immunoblotting (IB) with a general phosphotyrosine (PY; 4G10) antibody. (B) Lysates were immunoblotted with antibody to phosphorylated PLCγ2 (phospho-PLCγ2 [Tyr1217]), total PLCγ2 (n = 3), phosphorylated Akt (n = 3), total Akt (n = 3), phosphorylated p38 MAPK (phospho-p38), or total p38 (n = 3).
