Allogeneic antibodies develop 1 year after HCT. Plasma samples were obtained from a patient with AML 1, 2, 11, 12, 14, 16, and 18 months after transplantation and before transplantation, and from the donor. Raw data from protein microarrays were normalized and plotted, with posttransplantation results plotted on the y-axis against the pretransplantation measurements on the x-axis. The 2 proteins identified as targets were CHAF1b and NuSAP1 (labeled “C” and “N,” respectively) and were first detected 11 months after transplantation. For the diagonal lines, the x-axis denotes the pretransplantation log-normalized fluorescent intensity and the y-axis denotes the posttransplantation log-normalized fluorescent intensity.