Spleen Fpn1 gene and protein expression in KI mice. (A) Relative spleen Fpn1 gene expression in 10-week-old KI mice compared with WT sib pairs. The assay was performed on at least 5 animals for each strain. ***P < .001 versus WT. Bars represent SD in the analyzed animal groups. (B) Western blot analysis of Fpn1 protein in splenic macrophages isolated from the 3 Tfr2 models at 10 weeks of age and compared with WT. Arrows indicate the position of the marker proteins. (C) Western blot analysis of spleen Fpn1 and Ft L proteins in KI mice of different ages versus WT. d indicates days; and w, weeks. Arrows indicate the position of the marker proteins. Vertical lines indicate reposition of the same gel's image.