Molecular detection of the Tfr2 proteins in the targeted mice. (A) Western blot demonstrating lack of α-Tfr2 in liver and spleen of KO and LCKO-KI and persistence of the protein in KI mice. First lane indicates transfected α-Tfr2 used as positive control. Tissues from different animals per genotype are shown; anti-TFR2 (Alpha Diagnostics International) antibody was used. Black arrows indicate marker size. Vertical lines indicate reposition of the same gel's image. (B) Western blot demonstrating lack of β-Tfr2 in KI, KO, and LCKO-KI spleen. First lane indicates transfected β-Tfr2 used as positive control; anti-Tfr2 S-20 antibody (Santa Cruz Biotechnology) was used. Black arrows indicate marker size. (C) Western blot showing β-Tfr2 in WT and band absence in KI splenic macrophages. First lane indicates transfected β-Tfr2 used as positive control; anti-Tfr2 S-20 antibody (Santa Cruz Biotechnology) was used. Black arrows indicate marker sizes.