Thrombus formation in RAG1−/− mice is not affected in vitro and in vivo. (A) Platelets in whole blood from RAG1−/− mice form stable thrombi when perfused over a collagen-coated surface at a shear rate of 1000 s−1. Left panel shows representative phase-contrast images (40×/0.25 NA air objective). Right panel shows mean surface coverage by thrombi in RAG1−/− mice and WT controls (n = 5 per group). P > .05, 2-tailed Student t test compared with WT controls. (B) In vivo analysis of thrombus formation in RAG1−/− mice and WT controls (n = 10 per group). Mesenteric arterioles were treated with FeCl3 and adhesion, and thrombus formation of fluorescently labeled platelets were monitored by in vivo fluorescence microscopy. Representative images (top) and the time to vessel occlusion (bottom) are shown. Each symbol represents 1 animal, and horizontal bars depict means. The asterisk (top) indicates occlusion of the vessels. Scale bar equals 25 μm. P > .05, 2-tailed Student t test compared with WT control.
