Infarct volumes and functional outcomes 24 hours after focal cerebral ischemia in WT mice and RAG1^−/− mice lacking T cells and B cells, as well as RAG1^−/− mice reconstituted with splenocytes from OTI/RAG1 mice, 2D2/RAG1 mice, CD28^−/− mice, or PD1^−/− mice. (A) Brain infarct volumes from the different animals groups indicated 24 hours after tMCAO as measured by planimetry (n = 5-10 per group). Note that the protection from stroke in RAG1^−/− mice was overcome by the adoptive transfer of splenocytes. (B) Top panel shows neurologic Bederson score and bottom panel shows grip test from the different groups indicated as assessed at day 1 after tMCAO (n = 5-10 per group). *P < .05 and **P < .001, Bonferroni-corrected 1-way ANOVA compared with WT controls. Error bars represent SD.