Loss of dimethyl H4R3 leads to disruption of long-range chromatin interactions between HS2 and the β^maj-globin promoter in the globin locus. (A) Schematic representation of the mouse β-globin locus. The globin genes are indicated by red boxes and DNase I hypersensitive sites (HSs) are indicated by arrows. The predicted 3C BglII fragments from HS2 and the β^maj-promoter are shown in gray. (B) Shown is 3C analysis of the interaction between HS2/3 and the β^maj-promoter in the mouse β-globin locus in the vector control or shPRMT1-transduced clones treated with or without DMSO for 3 days. The predicted 250-bp product was then subcloned and analyzed by DNA sequencing. (C) A total of 3 independent 3C experiments were quantitated by densitometry. Shown are the mean ± SDM of 3 independent experiments.