Normal T-cell precursor frequencies in BM and circulation of DKO mice. BM cells (A) and peripheral blood cells (B) were stained with antibodies against lineage markers, Sca-1, CD117, CD127, CD27, and CD135. HSCs, MPPs, and CLPs were defined as lin⁻CD135⁻CD117^hiSca-1⁺, lin⁻CD27⁺CD135⁺CD117^hiSca-1⁺, and lin⁻CD27⁺CD135⁺CD127⁺CD117⁺Sca-1⁺, respectively. BM stainings are representative of 4 individual mice. Blood stainings are representative for 2 stainings of pooled cells from 2 mice each.