Absence of Fyn detection in lymphomatous tissue and in microdissected glomeruli from one patient with cHL-MCNS. (A) Analysis of Fyn expression by RT-PCR in normal PBMC, in lymphomatous tissue from patient no. 8 and a control patient with isolated cHL, in the PBMC of 2 patients with an I-MCNS relapse and in HRS cell lines. (B) Analysis of Fyn expression in microdissected glomeruli from patient no. 8 and a control patient without glomerular disease. Notably, transcripts for FAT and NP1 were easily detected in the microdissected glomeruli. (C) Immunohistochemistry analysis of Fyn protein in normal human kidney (left panel) and in a kidney biopsy specimen from patient no. 8 (right panel). Note the absence of Fyn protein from podocytes of patient no. 8.