Up-regulation of c-mip in lymph nodes of 8 patients with cHL-MCNS is restricted to HRS cells. (A) Immunohistochemistry analysis of c-mip in normal lymphoid tissues (thymus, lymphoid follicle of the spleen and lymph nodes). Note that c-mip is not seen in normal tissues, except in the transitional zone of node tissue and in the lymphoid follicle (indicated by arrows) at an original magnification of ×20. (B) Representative image showing c-mip in lymphomatous tissues from 2 patients with isolated cHL at an original magnification of ×20. HRS cells are indicated by arrows. Note that c-mip is undetectable in lymphomatous tissues from isolated cHL. (C) Representative expression of c-mip in lymphomatous tissues from 2 patients with cHL-MCNS at an original magnification of ×20. c-mip shows intense staining in cHL-MCNS, restricted to HRS cells. (D) Localization of c-mip in HRS cells from 4 patients with cHL-MCNS. No staining was detected in the HRS cells of 2 patients with isolated cHL at an original magnification of ×100. c-mip up-regulation in HRS cells was found in all patients with cHL-MCNS, whereas no immunostaining was observed for c-mip in 9 control cases with isolated cHL