Increased IFN-γ immune responses in CD8⁺ and CD20⁺ lymphocyte-depleted SIV-infected AGMs. SIV_sab IFN-γ cytokine release of PBMCs obtained from 12 SIV-infected sabaeus AGMs. PBMCs from 6 control Ab-treated AGMs (A,C) and 6 CD8⁺ and CD20⁺ lymphocyte-depleted AGMs (B,D) were stimulated with SIV_sab Gag and Env 15-mer peptide pools for 18 hours at 37°C. The values given represent the number of IFN-γ SFCs in 10⁶ PBMCs in response to stimulation with the Gag (A-B) or Env peptide pool (C-D). Responses were considered positive if their value was greater than 2 times the SD of the mean of the media control wells. All values were background corrected using the media controls. p.i. indicates postinfection.