CD8⁺ T-cell and NK-cell depletion in SIV-infected AGMs. Absolute CD8⁺ T cells in peripheral blood (A-B) and lymph nodes (C-D) and peripheral blood NK-cell (E-F) counts in 12 sabaeus African green monkeys (AGMs) infected intravenously with SIV_sab9315BR. Six AGMs received 1 subcutaneous dose of 10 mg/kg of the anti-CD8α mAb cM-T807 on day 0 before simian immunodeficiency virus (SIV) infection and 2 intravenous doses of 5 mg/kg cM-T807 on days 3 and 7 after infection to deplete CD8⁺ lymphocytes (B,D,F). These 6 animals also received 50 mg/kg anti-CD20 rituximab antibody intravenously at days −7, 14, and 35 after infection to deplete B cells. The other 6 animals received 1 intravenous injection of 50 mg/kg IgIV 7 days before infection to serve as control animals (A,C,E). The ↓ in panels B, D, and F indicate the injection of the CD8⁺ lymphocyte-depleting mAb. CD8⁺ T cells were gated as CD8⁺ CD3⁺ lymphocytes and NK cells as CD8⁺ CD3⁻ lymphocytes. Absolute CD8⁺ T-cell and NK-cell numbers were calculated from white blood cell counts. p.i. indicates postinfection.