Cellular activation of plasminogen: effect of LBS ligands. (A) Cortical neurons (105 cells/well) and (B) THP-1 cells (105/well) were incubated with 125nM Glu-plasminogen supplemented with various concentrations of ϵ-ACA (0-25mM) and 0.75mM CBS0065. Plasmin formation (●) was detected by measuring the release of p-nitroaniline. (C) THP-1 cells (105/well) were incubated with 125nM Glu-plasminogen supplemented with of 0 to 1μM anti–K1-LBS mAb 34D3 and 0.75mM CBS0065. (A-C) After detection of plasmin formation, the cells were washed twice with PBS and incubated with 0.75mM CBS0065 to detect cell-associated plasmin (□). Results are expressed as a percentage (mean ± SD; n = 3) of plasmin formation or of cell-associated plasmin activity in the absence of ϵ-ACA or mAb.
