Characterization of DC precursors in neonatal spleen. C57BL/6J mice were analyzed for the presence of dendritic-like cells (DCs) and hematopoietic cell subsets by flow cytometric analysis for expression of c-kit, Sca1, CD34, VCAM, Thy1.2, CD69, Flt3, CD11c, and CD11b. Forward scatter (FSC) and side scatter (SSC) analysis was used to gate out debris, and isotype controls were used to determine background binding (shown as density plots) and to set gates. Specific antibody binding is shown by dot plots, and the percentage of cells is indicated in gates. Results are representative of 3 separate analyses.