Expression of membrane proteins of reticulocytes and erythrocytes. (A) Immunoblots: Western blots of SDS-PAGEs of total cellular protein prepared from reticulocytes and erythrocytes probed with antibodies against the indicated proteins. Note the dramatic decrease of CD71, GLUT4, Na-K-ATPase α, ICAM4, and NHE1; decrease to a lesser extent of Duffy, Kell, GPA, and CD47; and increase of band 3, Rh, RhAG, XK, and GPC in erythrocytes compared with reticulocytes. (B) Flow cytometry: the ordinate measures the number of cells displaying the fluorescent intensity given by the abscissa. Dark lines indicate reticulocytes; light lines, erythrocytes. Note the decreased expression of Duffy, GPA, Kell, and CD47 and the increased expression of band 3 and GPC in erythrocytes compared with reticulocytes.