![Figure 1. A truncated active SphK2 (t-SphK2) is released into the supernatant of apoptotic Jurkat cells. (A-B) Jurkat cells remained as controls or were treated with Sts as described in “Induction and detection of apoptosis.” (A) Western analysis of SphK2 in subcellular fractions (nuclear [Nuc], membrane [Mem], cytosolic [Cyt]) or the cell supernatant (Sup). The histogram shows quantification of Western data as means ± SEM from 3 independent experiments. (B) SphK2 activity in Jurkat cell lysates (■) and cell supernatants (□). Data are means ± SEM from 4 independent experiments. *P < .05 compared with unstimulated controls. (C) Western analysis of SphK2 expression in whole-cell lysate (L) and supernatant (S) from Jurkat and SphK2-deficient Jurkat cells that were untreated or treated with Sts. (D) Blots show expression of SphK2 in cytosol and supernatants of control or UV-irradiated (10 mJ/cm2) Jurkat cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/17/10.1182_blood-2009-10-243444/4/zh89991051990001.jpeg?Expires=1722698681&Signature=t48uKaLqemF-yQZyb7zFuj~tmOmxQH4PLrR0JwrqeriPuZAQtYQHsPeniYlX3XQJKFJxtmDpbYggPDo~AtmrTj3sg~x3~XjP32JiD9Zz1uBJJC8D5552TYcqO7tuwv3shrMUgwa2DW24YCGpu0M2q9t4YAW3fIJCROcoPenTw4mXHMwTbjaFBiJUNzO~AawTlnk-PfBOljt9O5WpQsxKuV~R5E4jMMrU26ZbNPmZepLqt8PM9wjouR0SmoPKWI0y~6VMW4MsqqudiKBHPOpcpv2DmyotPpZczhFxeobPLvs8r~i4HhMoieMeNq5OxgpvFGDrcYWcSgzsAj1z~BFFUA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
A truncated active SphK2 (t-SphK2) is released into the supernatant of apoptotic Jurkat cells. (A-B) Jurkat cells remained as controls or were treated with Sts as described in “Induction and detection of apoptosis.” (A) Western analysis of SphK2 in subcellular fractions (nuclear [Nuc], membrane [Mem], cytosolic [Cyt]) or the cell supernatant (Sup). The histogram shows quantification of Western data as means ± SEM from 3 independent experiments. (B) SphK2 activity in Jurkat cell lysates (■) and cell supernatants (□). Data are means ± SEM from 4 independent experiments. *P < .05 compared with unstimulated controls. (C) Western analysis of SphK2 expression in whole-cell lysate (L) and supernatant (S) from Jurkat and SphK2-deficient Jurkat cells that were untreated or treated with Sts. (D) Blots show expression of SphK2 in cytosol and supernatants of control or UV-irradiated (10 mJ/cm2) Jurkat cells.
