American Society of Hematology

Figure 3

$Figure 3. The phenotype of LICs can change in the mice. The expression of CD14 is seen in the primary AML sample from sample 7(A). The sample was sorted, and 250 000 CD14+ cells were transplanted into 3 mice, but no graft was detected at 9 weeks. By contrast 250 000 CD14− cells gave rise to a graft at 9 weeks in 2 of 2 mice transplanted (B), indicating that the LICs were CD14−. All the human CD45+ cells derived from the CD14− fraction expressed CD14 (C). These CD14+ cells were capable of initiating a graft in secondary recipients (D), indicating that the LICs were now CD14. The human CD45+ cells in the secondary recipients were CD14+ (E).$

The phenotype of LICs can change in the mice. The expression of CD14 is seen in the primary AML sample from sample 7(A). The sample was sorted, and 250 000 CD14⁺ cells were transplanted into 3 mice, but no graft was detected at 9 weeks. By contrast 250 000 CD14⁻ cells gave rise to a graft at 9 weeks in 2 of 2 mice transplanted (B), indicating that the LICs were CD14⁻. All the human CD45⁺ cells derived from the CD14⁻ fraction expressed CD14 (C). These CD14⁺ cells were capable of initiating a graft in secondary recipients (D), indicating that the LICs were now CD14. The human CD45⁺ cells in the secondary recipients were CD14⁺ (E).

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