Figure 5
Different parameters affect the ability of polyP to modulate lysis. Fibrin clots were formed from fibrinogen (2.4μM), plasminogen (0.24μM), and tPA in the absence (○) and presence (●) of polyP65 (325μM) by adding thrombin and CaCl2 (A). The concentration of thrombin used to induce clotting was varied from 0.016 U/mL to 5 U/mL with constant CaCl2 (5mM) and tPA (20pM) concentrations. (B) The concentration of CaCl2 was varied from 0 to 20mM in the presence of constant thrombin (0.25 U/mL) and tPA (20pM) concentrations. (C) The tPA concentration was varied from 10 to 500pM in the presence of constant thrombin (0.25U/mL) and CaCl2 (5mM) concentrations. All the results are expressed as the mean ± SEM time to 50% lysis (n = 4).

Different parameters affect the ability of polyP to modulate lysis. Fibrin clots were formed from fibrinogen (2.4μM), plasminogen (0.24μM), and tPA in the absence (○) and presence (●) of polyP65 (325μM) by adding thrombin and CaCl2 (A). The concentration of thrombin used to induce clotting was varied from 0.016 U/mL to 5 U/mL with constant CaCl2 (5mM) and tPA (20pM) concentrations. (B) The concentration of CaCl2 was varied from 0 to 20mM in the presence of constant thrombin (0.25 U/mL) and tPA (20pM) concentrations. (C) The tPA concentration was varied from 10 to 500pM in the presence of constant thrombin (0.25U/mL) and CaCl2 (5mM) concentrations. All the results are expressed as the mean ± SEM time to 50% lysis (n = 4).

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