Figure 4
PolyP delays fibrinolysis. Fibrin clots were formed with purified fibrinogen (2.4μM) in the absence (gray line) and presence (black line) of polyP65 (325μM). Lysis was induced by including 20pM tissue plasminogen activator (tPA) and 0.24μM plasminogen (A) or 11nM plasmin (B) with fibrinogen before activation with thrombin (0.25 U/mL) and CaCl2 (5mM). Lysis was monitored by changes in absorbance at 340 nm for 4 hours with time points every 1 minute. Results are normalized to account for polyP-induced changes in turbidity and are expressed as mean ± SEM of the percentage of lysis (n = 6). (C) Lysis was followed as described for tPA and plasminogen but with the addition of various polyP65 concentrations (0-3.25mM). The time to mean time to 50% lysis ± SEM is shown (n = 3). (D) Clots were formed in the presence of monophosphate or polyphosphate of various chain lengths (5-65 phosphate residues) at equivalent monomer concentration (325μM). Lysis by tPA and plasminogen was monitored, and the mean 50% lysis time ± SEM is shown (n = 4). Fifty percent lysis times of statistical significance are denoted with **P < .001 and ***P < .001).

PolyP delays fibrinolysis. Fibrin clots were formed with purified fibrinogen (2.4μM) in the absence (gray line) and presence (black line) of polyP65 (325μM). Lysis was induced by including 20pM tissue plasminogen activator (tPA) and 0.24μM plasminogen (A) or 11nM plasmin (B) with fibrinogen before activation with thrombin (0.25 U/mL) and CaCl2 (5mM). Lysis was monitored by changes in absorbance at 340 nm for 4 hours with time points every 1 minute. Results are normalized to account for polyP-induced changes in turbidity and are expressed as mean ± SEM of the percentage of lysis (n = 6). (C) Lysis was followed as described for tPA and plasminogen but with the addition of various polyP65 concentrations (0-3.25mM). The time to mean time to 50% lysis ± SEM is shown (n = 3). (D) Clots were formed in the presence of monophosphate or polyphosphate of various chain lengths (5-65 phosphate residues) at equivalent monomer concentration (325μM). Lysis by tPA and plasminogen was monitored, and the mean 50% lysis time ± SEM is shown (n = 4). Fifty percent lysis times of statistical significance are denoted with **P < .001 and ***P < .001).

Close Modal

or Create an Account

Close Modal
Close Modal