Figure 1
PolyP binds to fibrinogen and soluble fibrin. (A) Fibrinogen (10 μg) was incubated with zirconia beads coated with either polyP65 or bovine serum albumin (BSA). The starting material (SM), flow-through (FT), low-salt (LS), and high-salt (HS) washes were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis on 4% to 12% NuPAGE gels with MOPS running buffer and blotted with an antibody to fibrinogen. (B) PolyP65 was incubated with 10 μg of soluble fibrin (SF), fibrinogen (FG), or buffer alone for 10 minutes before separating on 6% Tris-glycine gels for 2.5 hours under native conditions. The gels were stained with toluidine blue O that binds polyP. Free polyP65 migrates with the dye front and cannot be visualized.

PolyP binds to fibrinogen and soluble fibrin. (A) Fibrinogen (10 μg) was incubated with zirconia beads coated with either polyP65 or bovine serum albumin (BSA). The starting material (SM), flow-through (FT), low-salt (LS), and high-salt (HS) washes were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis on 4% to 12% NuPAGE gels with MOPS running buffer and blotted with an antibody to fibrinogen. (B) PolyP65 was incubated with 10 μg of soluble fibrin (SF), fibrinogen (FG), or buffer alone for 10 minutes before separating on 6% Tris-glycine gels for 2.5 hours under native conditions. The gels were stained with toluidine blue O that binds polyP. Free polyP65 migrates with the dye front and cannot be visualized.

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