IκKβ and p85 interact with hGMR-α. (A) Flow cytometric analysis to detect hGMR-α and hGMR-α-APVA after transfection of HEK293T cells. hGMR-α was detected with 4H1 antibody (open histograms). An isotype-matched control is shown for comparison (gray histograms). (B) hGMR-α, p85, and IκKβ detected in whole-cell lysates (WCL) from HEK293T cells expressing hGMR-α and in 4H1 immunoprecipitates (4H1 IP). (C) hGMR-α and p85 detected in WCL from HEK293T cells expressing hGMR-α or hGMR-α-APVA and in 4H1 immunoprecipitates (4H1 IP) of the same cells. Control IPs minus antibody (−Ab), or with IgG1 (IgG₁) are also shown. (D) hGMR-α and IκB detected in WCL from HEK293T cells expressing hGMR-α or hGMR-α-APVA, and in 4H1 immunoprecipitates (4H1 IP). Control IPs minus antibody (−Ab), or with IgG₁ are also shown.