Figure 2
Figure 2. The hβc FIΔ mutant signals through Akt and NFκB pathways. (A) FDB1 cells were cultured in the absence of growth factor for 16 hours and then stimulated with 500 BMU of mIL-3 or mGM-CSF for 5 minutes. Whole-cell lysates were subjected to Western blot analysis with the indicated antibodies. (B) FDB1 cells cultured in 500 BMU mIL-3 and FDB1 cells expressing FIΔ or V449E cultured in the absence of growth factor were treated with DMSO, LY294002 (LY), or wortmannin (Wort) for 24 hours. Viability was measured by 7-amino-actinomycin D staining and flow cytometry. Error bars represent SEM, where n = 2; statistical significance was calculated by the use of a Student t test, *P < .05 **P < .01.

The hβc FIΔ mutant signals through Akt and NFκB pathways. (A) FDB1 cells were cultured in the absence of growth factor for 16 hours and then stimulated with 500 BMU of mIL-3 or mGM-CSF for 5 minutes. Whole-cell lysates were subjected to Western blot analysis with the indicated antibodies. (B) FDB1 cells cultured in 500 BMU mIL-3 and FDB1 cells expressing FIΔ or V449E cultured in the absence of growth factor were treated with DMSO, LY294002 (LY), or wortmannin (Wort) for 24 hours. Viability was measured by 7-amino-actinomycin D staining and flow cytometry. Error bars represent SEM, where n = 2; statistical significance was calculated by the use of a Student t test, *P < .05 **P < .01.

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