Serial transplantation reduces CML-like disease. Recipients were transplanted with 1 × 106 ufBM cells or 2500 LSK cells, isolated from dtg or stg mice. Donors had either been induced for 3 weeks (preinduced) or never been induced (noninduced) before BM harvest. (A) FACS analyses of BM and spleen showed increasing percentages of immature granulocytes in mice that had been transplanted with either preinduced or noninduced ufBM or LSK cells from dtg mice. Graphs show mean ± SD; n = 4 per group. (B) Serial transplantation of 1 × 106 ufBM cells isolated from diseased primary recipients of ufBM (first-degree transplantation) did not lead to CML-like phenotype in BM of secondary recipients (second-degree transplantation). Depicted is the percentage of immature granulocytes in BM and spleen of serial transplanted mice; n = 4. (C) Splenomegaly was only evident in primary recipients but was not observed on serial transplantation. (D) Histologic analysis by NACE stain of the spleen of secondary recipients that had received ufBM from preinduced donors showed no alterations of spleen morphology. *P < .05.
