The nature of WM T-cell clonality from 1 representative WM patient (patient no. 4). (A) Flow cytometric cell sorting of CD3⁺CD4⁻TCRVβ⁺CD57⁺ and CD3⁺CD4⁻TCRVβ⁺CD57⁻ cells from PBMCs of WM patient no. 4. (B) CDR3 length profile of CD3⁺CD4⁻TCRVβ⁺CD57⁺ and CD3⁺CD4⁻TCRVβ⁺CD57⁻ cells using 6-FAM-Cβ and specific-Vβ20 primer for WM patient no. 4. (C) DNA sequence analysis in sorted cells from WM patient no. 4. The sequences are shown below the trace pattern.