Antiviral function of CB-derived MV-CTLs. (A) Expression of CAR.CD19 in transduced (top histogram) compared with nontransduced (bottom histogram) CB-derived MV-CTLs 1 week after transduction. (B-C) IFNγ production by CAR.CD19⁺ CB-derived MV-CTLs compared with nontransduced CTLs in response to CMV-pp65 and AdV-hexon pepmixes as measured by ELISPOT assay for 2 of the CB donors. (D-E) Specific lysis of CMV-pp65, AdV-hexon, and irrelevant pepmix-pulsed autologous PHA blasts, and autologous EBV⁺ CD19⁺ LCLs by CAR-transduced (left graphs for each panel) and NT (right graphs for each panel) CB-derived MV-CTLs at the indicated E/T ratios measured by ⁵¹Cr-release assay for 2 representative donors. TD CTLs indicates transduced cytotoxic T lymphocytes; and NT CTLs, nontransduced cytotoxic T lymphocytes.