Figure 2
Donor-derived BM progenitors contribute to all stages of thymopoiesis after their IT administration. (A) CD45.1+ WT lin− BM cells (2 × 105) were injected intrathymically in ZAP-70−/− mice. Thymi were harvested between 20 and 25 weeks after injection, and the percentages of donor CD45.1+ thymocytes in each thymocyte subset were analyzed. Representative histograms show the percentages of donor TN1, TN2, TN3, TN4, immature single-positive (ISP), CD3− DP, CD3+ DP, CD4 SP, as well as CD8 SP thymocytes. (B) Bar graph quantification showing the percentages of CD45.1+ donor thymocytes at each maturation stage are presented as means ± SDs (n = 11).
