Figure 6
SLP-76 and phospho-Vav1 localization in platelets captured onto fibrinogen from shear flow. (A) ADAP+/+ and ADAP−/− platelets were stained with an antibody against SLP-76 (green) and with rhodamine-phalloidin to label F-actin (red); yellow indicates colocalization. (B) Localization of Vav1 pTyr-174 in perfusion-fixed ADAP+/+ (top) and ADAP−/− (bottom) platelets costained with an antibody against GP IX. Note prominent Vav1 phosphorylation in ADAP+/+ platelets, but its almost complete absence in ADAP−/− platelets. Minimal nonspecific staining was seen with the use of a control rabbit IgG antibody. (C) Quantification of average Vav1 pTyr-174 fluorescence per platelet captured from flow. Results shown are the average of 3 experiments ± SEM. *P < .05.

SLP-76 and phospho-Vav1 localization in platelets captured onto fibrinogen from shear flow. (A) ADAP+/+ and ADAP−/− platelets were stained with an antibody against SLP-76 (green) and with rhodamine-phalloidin to label F-actin (red); yellow indicates colocalization. (B) Localization of Vav1 pTyr-174 in perfusion-fixed ADAP+/+ (top) and ADAP−/− (bottom) platelets costained with an antibody against GP IX. Note prominent Vav1 phosphorylation in ADAP+/+ platelets, but its almost complete absence in ADAP−/− platelets. Minimal nonspecific staining was seen with the use of a control rabbit IgG antibody. (C) Quantification of average Vav1 pTyr-174 fluorescence per platelet captured from flow. Results shown are the average of 3 experiments ± SEM. *P < .05.

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