![Figure 6. TNFR1 expression is not needed for T-cell priming in vivo. (A) Proliferation of 2.5 × 105 (gray and black bars) or 1.25 × 105 (□ and ) CD4+ T cells from either naive TNFR1+/+ or TNFR1−/− mice, or primed TNFR1+/+ or TNFR1−/− mice were stimulated for 72 hours with 5 × 105 of either unmodified ( and □) or TNCB-modified (■ and ) APCs; [3H] thymidine was added for the final 6 hours. (B) Frequency of IFN-γ–producing TNCB-specific CD4+ T cells, 5 × 105 (■), or 2.5 × 105 () T cells from either naive or sensitized TNFR1+/+ or TNFR1−/− mice were stimulated on anti–IFN-γ mAb-coated 96-well plates with 5 × 105 either unmodified APCs (, third column) or TNBS-modified APCs (■ and ). After 48 hours of incubation, ELISPOT assay was developed. Control T cells were stimulated with 10 μg/mL concanavalin A and APCs (□); 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/8/10.1182_blood-2008-11-187682/4/zh89990940510006.jpeg?Expires=1724941779&Signature=XzALJFlGkEpCSS-~MYEEoAq7VrAzkfTyWT6PaZH3JPhaFju8CQz7fviAFT52w3oUFDQIDbNocUIqiHsMnpmUPo3lNNGfwMMeL6lwAR7kK~pxMYiKdW-cM1vAJk4QLeogPuj7HnViW8KpuOc8ESd1HOrgp0dhUtFiCF2Sb~8i-EY4-FABQP1y4Ycqbz~m8DkIABkL-Oq9zNO-63a7uZAfw5cXvKfWmkPhgJ7O3bwW1dJzg0MaPhHHFhY3QP5Aw2KMVByDLLLbjVorVwv3kw6kMRy~7ECYVg2Hwg2Vt30rMfs~RoC8WqITJkn6LM158-80-IRCPIAD1HfcbgOUH08DyA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TNFR1 expression is not needed for T-cell priming in vivo. (A) Proliferation of 2.5 × 105 (gray and black bars) or 1.25 × 105 (□ and 
) CD4+ T cells from either naive TNFR1+/+ or TNFR1−/− mice, or primed TNFR1+/+ or TNFR1−/− mice were stimulated for 72 hours with 5 × 105 of either unmodified (
and □) or TNCB-modified (■ and ) APCs; [3H] thymidine was added for the final 6 hours. (B) Frequency of IFN-γ–producing TNCB-specific CD4+ T cells, 5 × 105 (■), or 2.5 × 105 () T cells from either naive or sensitized TNFR1+/+ or TNFR1−/− mice were stimulated on anti–IFN-γ mAb-coated 96-well plates with 5 × 105 either unmodified APCs (, third column) or TNBS-modified APCs (■ and ). After 48 hours of incubation, ELISPOT assay was developed. Control T cells were stimulated with 10 μg/mL concanavalin A and APCs (□); 3 independent experiments.
