A high-cholesterol diet is associated with MK delocalization toward BM sinusoids. (A) Hypercholesterolemia is associated with MK clustering on the BM vasculature lining (V), as detected by immunostaining for FVIIIra; bar, 100 μm. Greater magnification of BM smears clearly shows that MK are juxtaposed to the BM vasculature (V); bar, 30 μm. Ad, adipocytes. (B-C) Juxtavascular and disperse MKs and vessel count in BM smears, at low amplification, revealed that hypercholesterolemia is accompanied by an increase in juxtavascular and decrease in disperse MK. *P < .05. These quantifications were obtained in 2 independent experiments in BM smears from 3 mice/experimental group with consistent results.