Flow cytometric analysis of peripheral blood and bone marrow of ossicles shows the origin of the ossicle bone marrow. Ossicles were made subcutaneously in CD45.2 mice with BMSCs harvested from CD45.1 mice. No CD45.1 donor type hematopoietic cells were found in either the peripheral blood or ossicle bone marrows during the 16 weeks after the implantation period (n = 5). The chart is a representative analysis of ossicle bone marrow.