Forodesine treatment triggered an increase of p73 at mRNA and protein levels and the induction of FOXO1 and FOXO3a. (A-B) Cells from 4 representative patients with CLL were treated with forodesine (2 μM) and dGuo (10 and 20 μM) for 16 hours, and p53, p73, FOXO1, and FOXO3a proteins were analyzed by Western blot in total protein extracts. β-Actin was used as internal loading control. Densitometric protein quantification was performed, and p73, FOXO1, and FOXO3a fold inductions with respect to control were calculated. (C) Analysis of mRNA expression by quantitative reverse transcription polymerase chain reaction of p73 in primary cells from 3 patients with CLL treated for 4 to 6 and 8 hours with forodesine (2 μM) and dGuo (10 and 20 μM). mRNA relative levels are given as arbitrary units, using untreated cells as a reference control.