EphrinB is phosphorylated in developing, but not adult mouse retinal vessels. Retinal whole mounts from (A) adult (3-month-old) and (B) P6 mice were immunostained for CD31 (endothelial cells), NG2 (pericytes), and phospho (p)-EphrinB. Representative confocal images showing broad p-EphrinB detection in retinal vessels of P6, but not adult mice, overlapping with CD31 and partly NG2 immunostaining. Scale bars in panels A and B represent 20 μm. (C) p-EphrinB in retinal extracts from mice of different ages (from P6 to adult) detected by immunoblotting. The blot was stripped and reprobed for actin.