Palmitoylation-defective NRASD12^C181S retains the ability to bind Raf-1-RBD. RAS-GTP precipitated with glutathione-agarose beads bound with GST-fused RAS-binding domain (residues 1-149) of Raf-1 from serum-starved or unstarved NIH3T3 cells expressing GFP alone, NRASD12, nonpalmitoylable NRASD12^C181S, or nonprenylable NRASD12^C186S were analyzed by Western blotting with an anti–pan-RAS (RAS10) antibody. RAS from the same lysates was probed as a loading control.