HNP1-3 inhibit M-CSF–induced monocyte differentiation. (A) Healthy donor monocytes were incubated with 100 ng/mL rhM-CSF and increasing concentration of rhHNP3 for 2 days before immunoblot analysis of indicated proteins. (B-C) RQ-PCR analysis of CD163 and MCP1 gene expression in sorted CMML monocytes (CD14⁺/CD24⁻) exposed to 100 ng/mL rhM-CSF in the absence or presence of rhHNP3 for 6 days (B) and in healthy donor monocytes, and CD14⁺/CD24⁻ and CD14⁻/CD24⁺ CMML cells exposed to M-CSF and 5 μg/mL rhHNP3 for 1 to 4 days. (B-C) Values normalized to L32 mRNA level, expressed in arbitrary units. Mean ± SD of triplicates; 1 of 4 independent experiments is shown.