Synthesis and secretion of HNP1-3. (A-B) HNP1-3 expression in permeabilized healthy monocytes and neutrophils (CD16⁺) and CD14⁺/CD24⁻ (CD14⁺) and CD14⁻/CD24⁺ (CD14⁻) cells sorted from CMML. (A) One representative series of samples. (B) A series of 10 normal and 10 CMML samples was studied (mean fluorescence index reported to the control antibody; *P = .006; **P = .005; ***P = .001). (C) MS analysis of proteins secreted by CD14⁻/CD24⁺ (red lines) and CD14⁺/CD24⁻ (green lines) cells sorted from 3 representative CMML samples cultured for 6 hours in serum-free medium. (D) Immunoblot analysis of HNP1-3 in healthy donor (Co) CD14⁺ monocytes and CD16⁺ granulocytes, and in CD14⁻/CD24⁺, CD14⁺/CD24⁻, and CD16⁺ cells sorted from 2 CMML patients. (E) HNP1-3 was also examined by immunochemistry on cytospins of CD16⁺, CD14⁺/CD24⁻, and CD14⁻/CD24⁺ CMML cells and CD16⁺ granulocytes and CD14⁺ monocytes of healthy donors. (F) Correlation between HNP1-3 plasma level and the absolute number of granulocytes (including morphologically recognized polymorphonuclear cells and CD14⁻/CD24⁺ cells) in 47 patients with CMML.