Confocal-stacked image of a bone marrow megakaryocyte. hTg^G233V megakaryocytes were analyzed and show a typical granular distribution of VWF in the cytoplasm. Percoll-gradient megakaryocytes were fixed and labeled for VWF, CD41, and 4,6-diamidino-2-phenylindole. Maximum-intensity projection of the deconvolved image stack was obtained using a spinning-disk confocal microscope. Deconvolution was performed to get a sharper picture. A composite of VWF in red, CD41 in green, and nuclear staining in blue is shown. VWF is visualized as part of the megakaryocyte granule content, as opposed to CD41, which is most visible on the surface of the platelet precursor. No surface-bound VWF was observed.