Sequence conservation of the immediate upstream VEGFR1 promoter. Top part shows evolutionary conservation of the promoter region between −7 and −125 (chr13:27967,272-27967,390) using the UCSC Gene Browser. Blue/green tracks on the top part of the figure illustrate conservation of the sequence in primates (top tracks), placental mammals (middle tracks), and vertebrae (bottom, light blue/light green tracks) using the PhyloP and PhastCons packages (Cornell University), respectively. Conserved elements identified by PhastCons are shown as magenta (primates), purple (placental mammals), and brown (vertebrae) bars. Sequence alignments show conservation of the CRE motif at −81, the −52 and −39 ETS motifs, and the EGR binding site at −25. Although highly conserved, the −39 ETS site was not identified as a binding site by MultiTF.