Effect of 5-azaC on the expression of genes involved in regulation of immune response and cell cycle. (A) RNA expression of FOXP3, FOXO3a, IFN-γ, and IL-10 as evaluated by quantitative PCR. Exposure to 5-azaC significantly increased the expression of FOXP3 and FOXO3a and decreased the expression of IFN-γ and IL-10 at different time-points during the T-cell culture. (B) RNA expression of p16, p27, p53, and p73; a significant increase in the expression of all these genes was observed at different time points after exposure to the drug (n = 4 cases analyzed).